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Scanning Electron Microscope (SEM)

Electron Microscopes (EM) use a beam of accelerated electrons as source to interact with a sample to produce high definition magnified image. As the wavelength of electrons is much shorter (nearly 100,000 times) than that of visible light, the resolving power is very high for an EM in comparison to a light microscope and can reveal structures of very small objects down to nano-meter levels.


Scanning Electron Microscope

There are various types of EM such as:

  • Scanning Electron Microscope (SEM)
  • Transmission Electron Microscope (TEM)
  • Reflection Electron Microscope (REM)
  • Scanning Transmission Electron Microscope (STEM)
  • Scanning Tunneling Micorsopce (STM)

Scanning Electron Microscope (SEM) is one of the most important research tool which is used in all the fields of study and not just medical and biological sciences. In SEM, the images are produced when the accelerated electron beam scans the surface of the sample in a raster scan fashion. The electrons are generated by a source and they are incident on the sample to be imaged. These electrons interact with the electrons of sample thereby emitting electrons from the sample that are collected and processed to give an SEM Micrograph. Modern-day advanced SEMs can give a resolution of even 1nm and below.

Scanning Electron Microscope (SEM) is one of the most important research tool which is used in all the fields of study and not just medical and biological sciences. In SEM, the images are produced when the accelerated electron beam scans the surface of the sample in a raster scan fashion. The electrons are generated by a source and they are incident on the sample to be imaged. These electrons interact with the electrons of sample thereby emitting electrons from the sample that are collected and processed to give an SEM Micrograph. Modern day advanced SEMs can give a resolution of even 1nm and below.


SEM Image of an Insect Face at 57x Magnification